General Cell Based Assays
Eurofins Discoverx PathHunter eXpress SSTR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0308E2CP2M)
The PathHunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR4 and EA-tagged β-Arrestin-2. Activation of SSTR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Med Vet International Farrier Kit, J1024K, Each
Set of essential farrier equipment in an attractive black zipper case with carrying handles. • Hoof rasp with wooden handle • Farrier hammer • Standard hoof knife • Clinch cutter • Hoof nipper • Shoe puller • Weights 9.2 lbs • Measures 10" W x 1 3/4" D x 24" L
Eurofins Discoverx PathHunter eXpress EDNRB Total Internalization Assay, 2pl Kit. (93-0874E3CP0M)
The PathHunter EDNRB Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of EDNRB via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CALCRL-RAMP1 CHO-K1 GPCR Assay, 10-plate Kit. (95-0164E2CP2L)
The cAMP Hunter CALCRL-RAMP1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL (paired with RAMP1) GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP1. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MRGPRE CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0438E2ACP1L)
The PathHunter MRGPRE eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRE GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRE and EA-tagged β-Arrestin-2. Activation of MRGPRE stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GPR173 β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human GPR173 β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure GPR173 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR135 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR135 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR135 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress GPR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0335E2CP2L)
The PathHunter GPR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of GPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR1 and EA-tagged β-Arrestin-2. Activation of GPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 2-Plate Kit. (93-0746E3CP5M)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL12RB1/IL12RB2 Dimerization Assay, 2pl Kit. (93-1041E3CP0M)
The PathHunter IL12RB1/IL12RB2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin-1 GPCR Assay, 10pl Kit. (93-0974E2MCP2L)
The PathHunter mGLP1R (ARR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-1. Activation of mGLP1R stimulates the recruitment of β-Arrestin-1 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter PTH Bioassay Kit, 10-Plate. (93-0315Y2-00048)
The PathHunter PTH Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress PRLHR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0302E2CP2M)
The PathHunter PRLHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PrRP) based activation of PRLHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PRLHR and EA-tagged β-Arrestin-2. Activation of PRLHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences INDIGO Biosciences Activation Reporter Assay System, Human CHRM1, 96-well
This Human Cholinergic Receptor, Muscarinic 1 (CHRM1) Activation Reporter Assay System is a cell-based assay for screening compounds. It quantifies changes in luciferase activity to measure drug-induced changes in CHRM1 activity, identifying activators of CHRM1 or the coupled Ca+2-calcineurin / NFAT signal transduction pathway.
- Utilizes proprietary human cells engineered to constitutively express the human cholinergic receptor, muscarinic 1 (CHRM1).
- Measures drug-induced changes in CHRM1 activity through luciferase reporter gene expression.
- Designed for screening compounds that activate CHRM1 or the Ca+2-calcineurin / NFAT signal transduction pathway.
- Provided in a convenient 96-well format for high-throughput screening.
- Kit includes reporter cells, media, acetylcholine, detection reagents, and a collagen-coated assay plate.
Eurofins Discoverx PathHunter Human Heregulin beta 1 (ErbB2/ErbB4) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human Heregulin beta 1 (ErbB2/ErbB4) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of ErbB2/ErbB4. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.