General Cell Based Assays
Eurofins Discoverx PathHunter Mouse MDC (CCR4) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse MDC (CCR4) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mCCR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Revvity Health Sciences Inc HTRF Human IFN-α Detection Kit, 500 Assay Points
HTRF Human IFN-α Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Pro-Collagen Type 1 Detection Kit Standard
HTRF Human Pro-Collagen Type 1 Detection Kit Standard
Cell Signaling Technology PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
PathScanR Phospho-AMPK-alpha Thr172 Chemiluminescent Sandwich ELISA Kit 10 Kit
Abcam ATPase Assay Kit (Colorimetric)
ATPase Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter eXpress FPRL1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0044E2CP2M)
The cAMP Hunter FPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of FPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPRL1, a naturally Gi/Go coupled GPCR. Upon activation of FPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OSMR/IL6ST Dimerization Assay, 10pl Kit. (93-1056E3CP5L)
The PathHunter OSMR/IL6ST Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. oncostatin M) induced dimerization of OSMR/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside OSMR target protein fused to EA. Upon ligand-induced dimerization of OSMR/IL6ST, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL17RD/TNFR2 Dimerization Assay, 2pl Kit. (93-1068E3CP5M)
The PathHunter IL17RD/TNFR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TNFα) induced dimerization of IL17RD/TNFR2 receptors. This product uses EFC technology. The cell line included overexpresses IL17RD target protein fused to PK alongside TNFR2 target protein fused to EA. Upon ligand-induced dimerization of IL17RD/TNFR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR2 Total GPCR Internalization Assay, 10pl Kit. (93-0730E3CP0L)
The PathHunter HCRTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences LEADING LIGHT Wnt Reporter Assay Starter Kit (1x96wells)
Chemiluminescent cell-based assay for discovering Wnt pathway modulators. The LEADING LIGHT. .The assay has been used successfully in different assay formats (including HTS applications) to identify several distinct categories of small molecule compounds that modulate the Wnt signaling pathway. Alternative Name: Wnt reporter assay. Applications: FUNC. Chemiluminescence, HTS. Contents: LEADING LIGHT Wnt Reporter Cell Line, Cell Assay and Growth Medium Concentrate, Cell Freezing Medium, Luciferase Substrate and Assay buffer, HEPES, Wnt3a Protein (positive control), and Dkk-1 Protein
Indigo Biosciences Human Adrenoceptor Alpha 1D (ADRA1D) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1D reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1D. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Eurofins Discoverx cAMP Hunter LHCGR Bioassay Kit, 10-Plate. (95-0106Y2-00138)
The cAMP Hunter CHO-K1 LHCGR Bioassay Kit provides a robust, and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies.. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Montana Molecular Borealis Arrestin GCGR Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Glucagon receptor (GCGR) assay. Add the unmodified GCGR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, GCGR BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
Molecular Devices Fluorescent Assay Kit, cGMP, 96-Well, Competitive Immunoassay
The CatchPoint cGMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of 3',5'-cyclic guanosine monophosphate (cGMP) and guanylate cyclase activity in biochemical and cell-based assays. The kit provides sensitive fluorescence-based detection and includes pre-coated microplates and optimized reagents for high-throughput workflows.
- 96-well format with a black microplate and clear bottom.
- Includes reagents and buffers for assay setup and detection.
- Reagents sufficient for two 96-well plates (192 reactions at 120 μL).
- Fluorescence detection using Stoplight Red substrate, compatible with common plate readers.
- Typical sensitivity down to ~0.2 nM cGMP, dynamic range up to ~120 nM.
- Store kit components at 4°C; reconstituted working solutions stable for about three weeks at 4°C.
Eurofins Discoverx PathHunter eXpress P2RY1 Activated GPCR Internalization Assay, 2pl Kit. (93-0967E3CP0M)
The PathHunter P2RY1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.